ABSTRACT
Background: Preeclampsia (PE) is a pregnancy specific,hypertensive disorder. It affects 2-8% pregnancies.Oxidative stress and systemic inflammation areproposed to contribute significantly to the preeclampsiapathophysiology. The present study, aim is to determineand compare the markers of oxidative stress, endothelialdysfunction, systemic inflammatory markersNeutrophil/Lymphocyte Ratio (NLR) and Platelet/Lymphocyte Ratio (PLR) in preeclampsia andgestational age matched healthy controls. Material andMethods: This study was conducted in the Departmentof Biochemistry and Department of Obstetrics andGynecology, Sri Devaraj Urs Medical College, Kolar,Karnataka. The study included 98 preeclamptic womenand 98 normotensive pregnant women. Five ml venousblood was collected from all the study subjects. Bloodsample in EDTA vials was used for the complete bloodcount. NLR and PLR were calculated. Plasma was usedfor Ferric Reducing Ability of Plasma (FRAP) assay.Serum was used for the estimation of Malondialdehyde(MDA), nitric oxide, blood sugar, renal parameters andliver enzymes i.e., Aspartate Transaminase (AST),Alanine Transaminase (ALT), Lactate Dehydrogenase(LDH) and magnesium. Corresponding urine sampleswere collected for urinary protein analysis by dipstickmethod. Fetal outcome was recorded. Results:Gestational age was significantly low in preeclampticwomen as compared to those of controls. Bloodpressure (Systolic and diastolic), mean arterial pressure,body mass index, pulse rate, serum creatinine, uric acid,AST, ALT, LDH, MDA and NLR were increasedsignificantly in preeclamptic women as compared tothose of controls. In subgroup analysis, NLR wasincreased significantly in severe preeclamptics ascompared to mild preeclamptics. Serum Nitric Oxide(NO) and FRAP levels were decreased significantly inpreeclamptic women as compared to those of controls.Significantly decreased birth weight was observed inbabies born to preeclamptic mothers compared withcontrols. Conclusion: The present study resultsconclude that increased oxidative stress in termsincreased MDA, decreased NO and reduced antioxidantstatus (FRAP) in preeclamptic women, results inadverse perinatal outcome. In addition, maternal NLRcould be considered as a marker for severity ofpreeclampsia
ABSTRACT
Aim: To determine maternal thyroid hormone status and lipid profile in preeclampsia. Study Design: A prospective case control study consist of two groups such as group 1 normotensive non-preeclamptic group and group 2 as cases with clinically diagnosed preeclampsia Place and Duration of Study: Department of Obstetrics and Gynaecology, RL Jalappa Hospital and Research Centre kolar, between January 2012 and June 2013. Methodology: A total number of 100 pregnant patients were enrolled in the present study. Amongst, normotensive and preeclamptic pregnant women Group 1 (n=50) as controls (n=50). Group-2 (n=50) were preeclampsia cases. Five ml of blood samples were collected from each normal pregnant and preeclampsia patients. Thyroid hormone levels and lipid parameters were estimated using spectrophotometric method. Statistical analysis carried out by using Mean ± SD, and ‘t’ test. Results: The Mean ± SD values of T3 ng/ml (1.56±0.38), T4 (1.37±0.36) mcg/dl, TSH (2.45±1.23) mcIU/ml in normal pregnants and T3 ng/ml (1.37±0.36), T4 (9.87±2.83) mcg/dl, TSH (6.15±5.51) mcIU/ml in preeclampsia cases were presented. Similarly Mean ± SD values of total cholesterol (181.62±44.33) mg/dl, HDL cholesterol (49.44±11.54) mg/dl, Triglycerides (198.10±49.84) mg/dl, LDL cholesterol (104.90±29.58) mg/dl, and Total cholesterol/HDL cholesterol ratio (3.67) were from control group and total cholesterol (222.60±70.07) mg/dl, HDL cholesterol (45.92±11.81), Triglycerides (278.66±93.46) mg/dl, LDL cholesterol (116.52±49.26) mg/dl, and cholesterol and HDL cholesterol ratio (4.84) in preeclampsia were presented respectively. Conclusion: Pre eclampsia patients have higher levels of TSH and lower levels of T3 and T4 in comparison to normotensive pregnant women. This changes in the thyroid hormones did not correlate with the severity of preeclampsia between mild and severe pre eclampsia groups. Dyslipidemia was observed in pre eclampsia group than in the normotensive group indicates the possible atherogenic potential. This association may be useful in understanding the pathologic processes of preeclampsia.
ABSTRACT
Fluoride in cellular respiratory processes and its association in free radical generation are extensively studied. Cell permeability generates the oxidative stress through free radical species entry that sequentially affects the cellular biomolecules and cause toxic effects. Present study intended to determine the trend of oxidative stress and antioxidant status in the fluoride affected area that may raise the need for supplementation of antioxidants. Oxidative stress denoted in terms of ischemia modified albumin and antioxidants by vitamin C & total antioxidant capacity. Methods: Sixty subjects were divided into two groups. Group I (control) from fluoride unaffected areas (n=30) and group II subjects from fluoride affected area (n=30). Serum was assayed for Ischemia Modified Albumin (IMA), plasma for Total Antioxidant Capacity (TAC) and vitamin C. Results: The mean ± SD of IMA, TAC, and vitamin C of individuals subjected in fluoride affected areas are 2.403 ± 0.543, 210 mmol/l ± 82.2 and 0.62 mg/dl ± 0.23 respectively. The values were compared to individuals from fluoride unaffected areas have IMA (1.054 ABSU ± 0.851), TAC (312 mmol/l ± 62.1) and vitamin C (0.93 mg/dl ± 0.14) with P <0.001. Conclusion: The present study revealed that, individuals subjected to fluoride affected areas have more oxidative stress and decreased levels of antioxidant status indicates the risk of generation of free radicals intensity as a chief component of oxidative stress. This research outcome necessitates dietary supplementation of nutrient antioxidants to minimize the consequences of oxidative damage to vital biomolecules in fluoridated affected areas.